栗蚕幼虫酯酶同工酶酶谱多态性及应用于品种间亲缘关系的分析

Zymogram Polymorphism of Camphor Silkworm Larval Esterase Isozyme and Its Application in Analysis of Genetic Relationship Between Varieties

栗蚕是珍稀的野蚕资源。采用聚丙烯酰胺凝胶电泳法,测定栗蚕幼虫不同发育时期、不同组织器官的酯酶同工酶酶谱,并依据酯酶同工酶酶谱的多态性分析纯黑、纯绿2个品种的亲缘关系。检测结果表明:栗蚕幼虫中肠的酯酶同工酶活性和酶带数随龄期而增加,且同一龄期以盛食期的酶表达量最高;幼虫中肠及脂肪体中的酯酶同工酶活性较强,并且酶带数较多;2个供试品种间的酯酶同工酶酶谱明显不同。根据酶谱差异分析2个品种的遗传相似系数为0.86,表现出较近的亲缘关系。研究结果提示:栗蚕幼虫酯酶同工酶的酶活性和酶带数与生长发育和组织功能有关,幼虫酯酶同工酶酶谱可以作为分析品种间亲缘关系的依据之一。

Camphor silkworm（Dictyoploca japonica） is a rare silkworm resource.In present study,the zymograms of esterase isozymes during different developmental stages and in various tissues and organs of camphor silkworm larvae were determined using polyacrylamide gel electrophoresis.Moreover,the genetic relationship between two varieties named Chunhei and Chunlü was analyzed based on polymorphism of the esterase isozyme zymograms.The results showed that the activity of esterase isozymes and the number of enzymatic bands from midgut of camphor silkworm larvae increased with the advance of larval development,and the highest expression of isozymes was at the full appetite stage within the same instar.Higher activity of esterase isozymes and higher number of enzymatic bands were observed in midgut and fat body of the larvae.Zymograms of esterase isozymes from the two tested varieties were obviously different.Analysis on difference of the zymograms yielded a genetic similarity coefficient of 0.86 between the two varieties,showing relatively close genetic relationship.The obtained results suggest that the activity of esterase isozymes and number of enzymatic bands are related to growth,development and tissue functions of the camphor silkworm larvae.Therefore,esterase isozyme zymogram of camphor silkworm larvae can be used as a reference to analyze genetic relationship between different varieties.