利用泰乐菌素菌渣生产复合酶制剂及效果研究

Production of Complex Enzyme by Using Tylosin Dregs and Its Feeding Effect

发酵法生产抗生素过程中会产生大量废弃菌渣,这些菌渣因有机质含量高,堆放极易发生霉变,给当地环境造成危害。文章采用固体发酵法,以微生物降解处理后的泰乐菌素菌渣为主要原料,生产复合酶制剂,并对其在肉鸡上的使用效果进行研究。结果表明：在水含量50%～60%、干物质中麸皮含量不低于40%的泰乐菌素菌渣培养基中,接入5%~10%黑曲霉,在温度30℃、湿度60%~65%、培养基厚度10~14 cm条件下,好氧发酵60~72 h,可得到富含纤维素酶、木聚糖酶、β-葡聚糖酶、果胶酶和酸性蛋白酶的复合酶制剂。经动物饲喂试验,该酶制剂能显著（P〈0.05）提高肉鸡体增重和饲料转化效率,同时,肉鸡肌肉、肝脏和肾脏等组织中未检测到残留的泰乐菌素。

A mass of microbial dregs would be produced during antibiotic production by fermentation.The stacking dregs are liable to go mouldy because of high concentration of organic matter,and then cause the pollution of environment.In the present study,tylosin dregs after treated with microorganism were used as the main materials to prepare complex enzyme（CE） by solid fermentation,and the feeding effect of CE was also investigated.Aliquot 5% ~10% Aspergillus niger suspension was inoculated to tylosin dregs medium containing more than 40% wheat bran in dry matter.The content of water in medium was 50%~60%.CE,which contained amply cellulose,xylanase,β-glucanase,pectinase and acid protease was obtained by fermenting the inoculated medium for 60~72 h with conditions of temperature 30 ℃,humidity 60%~65% and medium thickness 10~14 cm.Data from the feeding experiment showed that CE significantly increased the body weight and feed efficiency of broilers.Tylosin residues in muscle,liver and kidney of broilers fed on the diet with CE were not detected.