刺参中无机元素的聚类分析和主成分分析

Principal Component Analysis and Cluster Analysis of Inorganic Elements in Sea Cucumber Apostichopus Japonicus

探讨多元素分析对中国刺参产地溯源的可行性,筛选判别刺参产地的有效指标。采用电感耦合等离子体质谱(ICP-MS)法测定了7个产地刺参样品中Al,V,Cr,Mn,Fe,Co,Ni,Cu,Zn,As,Se,Mo,Cd,Hg和Pb共15种无机元素的含量,建立刺参无机元素数据库。原始数据经过标准化处理后,结合聚类分析和主成分分析,对不同产地刺参进行了区分。主成分分析选出三个主因子,聚类分析将26个刺参样品聚成五大类,分类结果与刺参样品的海域分布关系显著。聚类分析和主成分分析是刺参无机元素分析的有效方法,刺参中无机元素的含量可做为刺参产地判别的测量指标之一。

The present study is to investigate the feasibility of multi-elements analysis in determination of the geographical origin of sea cucumber Apostichopus japonicus,and to make choice of the effective tracers in sea cucumber Apostichopus japonicus geographical origin assessment.The content of the elements such as Al,V,Cr,Mn,Fe,Co,Ni,Cu,Zn,As,Se,Mo,Cd,Hg and Pb in sea cucumber Apostichopus japonicus samples from seven places of geographical origin were determined by means of ICP-MS.The results were used for the development of elements database.Cluster analysis（CA） and principal component analysis（PCA） were applied to differentiate the sea cucumber Apostichopus japonicus geographical origin.Three principal components which accounted for over 89% of the total variance were extracted from the standardized data.The results of Q-type cluster analysis showed that the 26 samples could be clustered reasonably into five groups,the classification results were significantly associated with the marine distribution of the sea cucumber Apostichopus japonicus samples.The CA and PCA were the effective methods for elements analysis of sea cucumber Apostichopus japonicus samples.The content of the mineral elements in sea cucumber Apostichopus japonicus samples was good chemical descriptors for differentiating their geographical origins.