摘要
目的通过复制"两次打击"SIRS大鼠模型,探讨迷走神经切断及烟碱预处理对大鼠SIRS的作用,为临床治疗SIRS提供新思路。方法选择雄性健康SD大鼠40只,随机将40只实验动物分为4组,每组10只,对照组未予处理;单侧迷走神经切断组:在诱导SIRS模型前7d,行右侧颈部迷走神经切断;烟碱预处理组:在诱导SIRS模型前7d,开始每天给予烟碱液5mg.kg-1ip;联合迷走神经切断+烟碱预处理组:在诱导SIRS模型前7d,联合迷走神经切断+烟碱预处理。造模前晚禁食,不禁水,术日采用戊巴比妥钠腹腔注射麻醉,无菌条件采用腹颈正中切口显露右侧颈部迷走神经主干。比较4组大鼠TNF-a、IL-6含量。结果迷走神经切断后能提高炎症反应,烟碱预处理后能降低炎症反应,联合二者处理后炎症反应介于两者之间,且与未行迷走神经切断和烟碱预处理时炎症反应应答能力亦存在差别。结论中枢神经能够通过副交感神经途径调节炎症反应;烟碱具有较强的抗炎作用,其可不依赖于迷走神经的完整性而独立发挥作用。
Objective By copying the ＂two-hit＂ SIRS rats model of vagotomy and nicotine pretreatment on the mechanism of SIRS provide new ideas for the treatment or more effective treatment approach for clinical.Methods 40 male SD rats were randomly divided into A,B,C,D four groups,n = 10,A group（Control group） induced SIRS model in the first 7 days,not treated;B group（Unilateral vagotomy group）：induction of SIRS model in the first 7 days of right vagus nerve;C group（Nicotine pretreatment group）：induction of SIRS model in the first 7 days and given nicotine liquid（5mg/kg） intraperitoneal injection;D（Combined vagotomy ＋ Nicotine pretreatment group） in the 7 days before induction of SIRS model,combined vagotomy ＋ nicotine pretreatment last night,modeling,fast,can not help but water,day surgery with intraperitoneal injection of sodium pentobarbital anesthesia,sterile conditions by abdominal incision exposed the right side of the middle cervical vagus nerve trunk,left common carotid artery when modeling calculated for monitoring mean arterial pressure,blood letting and collection of blood samples;catheter for monitoring,to measure the mean arterial pressure,bleeding and collection of blood samples;the right common carotid artery catheter for blood transfusion fluid resuscitation.Bleeding from the left common carotid artery within 20min to the MAP to 50-60mmHg,30min after the maintenance of blood stored in heparin-vitro,within 120min after the transfusion of blood and equal ringer lactate.Comparison the four groups of rats with TNF-a,IL-6 levels.Results Vagotomy can increase inflammation,nicotine pretreatment can reduce inflammation,joint vagotomy ＋ nicotine pretreatment inflammation somewhere in between,and with no line of vagotomy and nicotine pretreatment differences in the ability of inflammatory response.Conclusion Central nervous system can adjust the inflammation through parasympathetic pathway;has strong anti-inflammatory effects of nicotine,which do not rely on the integrity of the vagus nerve function independently.
出处
《实用心脑肺血管病杂志》
2011年第11期1871-1874,共4页
Practical Journal of Cardiac Cerebral Pneumal and Vascular Disease
