眼针对腹泻型肠易激综合征模型大鼠结肠P物质表达的影响

Effects of eye-acupuncture therapy on expression of SP rats with irritable bowel syndrome

目的:观察眼针对腹泻型肠易激综合征(D-IBS)模型大鼠结肠组织中P物质(SP)表达的影响,探讨眼针治疗D-IBS的作用机制。方法:将30只SPF级雄性Wistar大鼠随机分为3组,每组10只:对照组、IBS组和眼针组。采用慢性应激结合束缚方法建立D-IBS模型。然后采用眼针治疗。取下焦区、大肠区、肝区、脾区进行针刺,治疗7d。采用ELISA测定血清和结肠组织中SP含量的变化;采用RT-PCR测定结肠组织SP mRNA表达变化;采用免疫组化测定结肠组织SP蛋白表达变化。结果:与对照组比较,IBS组血清和结肠组织中SP含量显著升高(P<0.01),结肠组织中SP mRNA及蛋白表达水平显著升高(P<0.01,P<0.05);与IBS组比较,眼针组血清和结肠组织中SP含量显著降低(P<0.01),结肠组织中SP mRNA及蛋白表达水平显著降低(P<0.01,P<0.05)。结论:眼针能够降低IBS模型大鼠血清和结肠组织中SP的含量及结肠组织中SP mRNA及蛋白表达,从而对IBS起到治疗作用。

Objective： To observe effects of the eye-acupuncture therapy on expression of SP in colon in rats with irritable bowel syndrome,in order to explore mechanism of action the eye-acupuncture therapy on IBS;Methods： 30 male,SPF grade,wistar rats were randomly divided into 3 groups： control group,IBS model group,eye-acupuncture group.IBS rat model was established by chronic stress combined with constraint.Eye-acupuncture group were treated by eye-acupuncture 7d.Detect SP content changes by ELISA in serum and colon.SP mRNA expression level was detected by RT-PCR.SP protein expression was detected by immunohistochemical method.Results： IBS model rats compared with normal control group SP were significantly elevated in serum and colon,expression of SP were significantly elevated eye-acupuncture group and Pinaverium bromide group compared with the IBS model group,SP were significantly lower in Serum and colon（P〈0.01,P〈0.05）.Conclusion： Eye-acupuncture can reduce expression of SP in colon in IBS rat,which played a therapeutic role in IBS.