当归-桃仁药对配伍特点及其效应物质基础研究

Study on the correlation of chemical constituents and activities of Radix Angelicae Sinensis and Semen Persicae

目的:探讨当归-桃仁药对不同配比的应用特点及其效应物质基础。方法:基于自建的中医方剂数据库,采用关联规则分析方法,挖掘归纳出当归-桃仁药对在方剂中的配伍特点;采用HPLC法分析当归-桃仁药对共煎液与单煎液不同配比中苦杏仁苷、绿原酸、咖啡酸、阿魏酸的含量变化;采用凝血时间、抑制血小板聚集和清除DPPH自由基实验评价配伍效应及其作用特点。结果:挖掘归纳出当归-桃仁药对在方剂中的配伍特点及其出现频度;实验结果表明当归-桃仁配伍有利于苦杏仁苷、绿原酸、咖啡酸、阿魏酸等效应成分的溶出;桃仁当归配伍比同等生药量的桃仁单味药材凝血时间延长,血小板聚集率抑制率增加,DPPH清除率增加;当归桃仁配伍比同等生药量的当归单味药材血小板聚集率抑制率增加,说明两者配伍具有一定的科学性和合理性。结论:当归-桃仁药对具有合理性,有利于两味药中芳香酸类化合物绿原酸、咖啡酸、阿魏酸及苷类化合物苦杏仁苷的溶出;配伍后凝血时间延长,血小板聚集率抑制率增加和DPPH清除率增加,但各配伍比例之间差异不明显。

Objective： Study on the correlation of chemical constituents and activities in different proportions of Radix Angelicae Sinensis and Semen Persicae.Methods： Data mining technology was applied on prescription to screening the proportions of Radix Angelicae Sinensis and Semen Persicae.HPLC determined the changes of the contents of amygdalin,chlorogenic acid,caffeic acid and ferulic acid in the single and mixed decoction of Radix Angelicae Sinensis and Semen Persicae.The experiments of thrombin time,anti-platelet aggregation and scavenging DPPH were evaluated the activities.Results： The top 10 proportions of Radix Angelicae Sinensis and Semen Persicae were screening.The contents of amygdalin,chlorogenic acid,caffeic acid and ferulic acid were higher than the single decoction of Radix Angelicae Sinensis and Semen Persicae.Thrombin time was prolonged,anti-platelet aggregation was increased and the activity of scavenging DPPH was enhanced when compared to the same drug dosage of the single decoction of Semen Persicae.The activity of anti-platelet aggregation was also increased when compared to the same drug dosage of the single decoction of Radix Angelicae Sinensis.Conclusion： Mixed decoction of Radix Angelicae Sinensis and Semen Persicae was rationality.The change of chemical constituents and activities has some correlation,but different proportions have no conspicuous variability.