摘要
目的:探讨柳叶蜡梅提取物在体外对人胃癌细胞株SGC-7901的增殖抑制作用及其可能的机制。方法:应用细胞培养、噻唑蓝(MTT)比色法、光镜观察、扫描和透射电镜观察、Hoechst33258/PI染色等技术研究柳叶蜡梅提取物对SGC-7901细胞的抑制作用。结果:柳叶蜡梅提取物可明显抑制SGC-7901细胞生长,随着药物浓度的增加,呈现剂量依赖性效应;光镜及扫描透射电镜观察显示,药物处理组细胞表面微绒毛减少并发泡、细胞核固缩、染色质凝聚等形态学特征;细胞核经Hoechst33258染色出现颗粒状荧光,而PI染色无红色荧光出现。结论:柳叶蜡梅提取物对人胃癌SGC-7901细胞具有明显的抑制作用,其机制可能与细胞凋亡相关。
Objective: To investigate the inhibitory effect of extract of Chimonanthus salicifolius on human gastric cancer cell line SGC-7901 and the related mechanism.Methods: The effects of extract of Chimonanthus salicifolius on the cell proliferation of SGC-7901 cells were determined with MTT,light microscopy,electron microscopy,Hoechst 33258/PI staining.Results: The results showed that extract of Chimonanthus salicifolius treatment could significantly inhibit the growth of SGC-7901 cells in a dose-dependent manner.Light microscopy and electron microscopy showed that the morphology of the treated cells appeared less microvilli,blebbing,karyopyknosis,chromatin condensation.Cell nucleus concentrated and appeared granular fluorescence by Hoechst 33258 staining.Conclusion:This study suggested that Chimonanthus salicifolius has significant inhibitory effects on SGC-7901 cells proliferation,the possible mechanism might be related to cell apoptosis.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2011年第10期2420-2422,共3页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
浙江省丽水市市级重点项目(No.20064011)~~
关键词
柳叶蜡梅提取物
胃癌
细胞增殖
Extract of Chimonanthus salicifolius
Gastric cancer
Cell proliferation