摘要
目的建立液相色谱—串联质谱法(LC-MS/MS)测定血浆中盐酸曲美他嗪浓度的方法,并研究其药动学行为。方法选取我院2007年2月至2009年2月18名健康成年人作为药物试验对象,均单剂量口服盐酸曲美他嗪40mg后,采用液相色谱—串联质谱法(LC-MS/MS)测定血药浓度,应用AIC法判断房室模型,并利用DAS软件计算药动学参数。结果主要药动学参数:Cmax为(126.11±20.18)μg/L,tmax为(1.78±0.65)h,t1/2Ke为(5.87±0.79)h,Ke为(0.13±0.03)h,V/F为(4.00±0.59)L/kg,CL/F为(0.49±0.09)L.h-1.kg-1,AUC0-24为(1208.79±198.36)μg.h-1.L-1,AUC0-∞为(1304.46±224.13)μg.h-1.L-1。结论该分析方法简单、快速、准确、精密度高,适用于人体中盐酸曲美他嗪浓度的测定。盐酸曲美他嗪的血浆药动学参数与国内外文献报道基本一致。
Objective To establish an LC-MS/MS method for the determination of trimetazidine hydrochloride in human plasma,and to study its pharmacokinetics.Methods Volunteers received a single oral dose of 40mg trimetazidine hydrochloride tabletsand the plasma concent ration of trimetazidine hydrochloride was differentiated by LC-MS/MS method.The compartment model was differentiated by AIC method and DAS program was used to calculate the pharmacokinetic parameters.Results The drug to the main pharmacokinetic parameters：Cmax of（126.11±20.18）μg/L,tmax was（1.78±0.65）h,t1/2Ke of（5.87±0.79）h,Ke of（0.13±0.03）h,V/F was（4.00±0.59）L/kg,CL/F of（0.49±0.09）L·h-1·kg-1,AUC0-24 of（1208.79±198.36）μg·h-1·L-1,AUC0-∞ of（1304.46±224.13）μg·h-1·L-1.Conclusion The method is suitable to determine theconcent ration of trimetazidine hydrochloride in human plasma because it is rapid and sensitive.The pharmacokinetic parameters of trimetazidine hydrochloride are similar to those reports home and abroad.
出处
《中国现代医药杂志》
2011年第9期58-59,共2页
Modern Medicine Journal of China
