水稻BWMK1互作基因的分离

Identification of rice genes interacting with BWMK1

为探明BWMK1介导的信号传导途径，采用酵母双杂交系统，构建水稻稻瘟病菌诱导后的cDNA文库，以BWMK1为诱饵，对文库进行筛选，通过验证、测序和基因编码分析，获得了7个BWMK1互作基因BWIPI-BWIP7，分别位于水稻基因组第12、2、3、10、6、4、5染色体上；7个互作基因中，除BWIP2和BWIP7未找到同源编码蛋白外，其余5个基因BWIP1编码硫甲基转运酶，BWIP3编码磷酸肌醇合成酶，BWIP4编码磷转运蛋白，BWIP5编码WD-40重复包含蛋白，BWIP6编码N-乙酰谷氨酸激酶。

The rice MAPK gene BWMK1 is transcriptionally activated by fungal pathogen infection and wounding and acts as a positive regulator in plant disease resistance signaling. To further understand the BWMKl-mediated molecular signaling regulating pathways, a yeast two-hybrid system using BWMK1 as a bait was used to screen BWMK1 interacting proteins, and 7 BWMK1 interacting protein encoding genes BWIP1-BWIP7 were isolated, BWIP1-BWIP7 were located on 12, 2, 3, 10, 6, 4, 5 chromosomes, respectively. Homologous coding proteins were found for each isolated gene except BWIP2 and BWIP7, which indicated that BWIP1, 3, 4, 5 and 6 respectively coded for 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase, myo-inositol phosphate synthease, putative phosphate transporter, WD-40 repeat containing protein and N-acetyl glutamate kinase 2.