茯砖茶水提物对大肠杆菌感染小鼠的免疫调节作用

Immunological effect of water extract from Fuzhuan brick-tea on mice infected with Escherichia coli

以肠出血性大肠杆菌O157:H7感染的小鼠为模型,研究造模后按茯砖茶水提物剂量1、10、20 g/(kg.d)(分别记为低、中、高剂量茶汁组)连续灌胃10 d后模型小鼠免疫功能的变化。结果表明,模型组和对照组的吞噬系数、血清溶血素水平和肠道黏膜上CD4+T、CD8+T淋巴细胞数均有显著性差异;中剂量茶汁组小鼠胸腺指数和高剂量茶汁组小鼠血清溶血素水平均极显著高于模型组小鼠(P<0.01),中剂量茶汁组小鼠血清溶血素水平和高剂量茶汁组小鼠胸腺指数以及中、高剂量茶汁组小鼠肠道黏膜上CD4+T、CD8+T淋巴细胞数均显著高于模型组小鼠(P<0.05);中、高剂量茶汁组小鼠的吞噬系数极显著高于模型组小鼠(P<0.01),低剂量茶汁组小鼠的吞噬系数显著高于模型组小鼠(P<0.05)。

Aimed to investigate the effect of water extract from Fuzhuan brick-tea on the immune functions of mice, one hundred and ninety mice were divided into 5 groups： control group, model group in which the mice were infected with Escherichia coli O157：H7, low dosage group, moderate dosage group and high dosage group in which mice were treated with low, moderate and high dosage of water extract from Fuzhuan brick-tea respectively for consecutive 10 d. Results demonstrated that there is remarkable difference in the phagocytic index, the serum hemolysin titers and the number of CD4＋ and CD8＋ T-lymphocytes in intestinal mucosa between control and model group. Compared with model group, the thymus index in moderate group and serum hemolysin titers in high dosage group are significantly higher （P 〈 0.01） ; the serum hemolysin titers in moderate group and the thymus index in high dosage group and the number of CD4＋ and CD8＋ T-lymphocytes in intestinal mucosa in moderate and high dosage groups increased significantly （P〈0.05）; the phagocytic index increased significantly in low （P 〈 0.05）, moderate and high （P 〈 0.01） dosage groups.