玉蜀黍平脐蠕孢拮抗细菌YB01的鉴定及拮抗作用

Identification of the antagonistic bacterium YB01 against Bipolaris maydis and its antagonism

通过形态特征、生理生化及16S rDNA同源性序列分析对玉蜀黍平脐蠕孢(Bipolaris maydis)拮抗细菌YB01进行鉴定,结果表明,该菌为苍白杆菌属的一种(Ochrobactrumsp.),序列号为HQ141339。YB01对玉蜀黍平脐蠕孢的拮抗作用研究表明:YB01培养液可以有效抑制玉蜀黍平脐蠕孢的生长、孢子的形成与萌发。显微镜观察结果表明,用YB01培养液处理的玉蜀黍平脐蠕孢菌丝体膨大变形,细胞质发生浓缩,表明YB01的拮抗作用主要是通过其代谢产物影响玉蜀黍平脐蠕孢细胞壁而实现的。

The antagonistic bacterium YB01 was identified by the characteristics of morphology, physiology, biochemical tests and sequence analysis of 16S rDNA, which was identified as Ochrobactrum sp. The antifungal mechanism of the YB01 was investigated in this study. The results indicated that mycelial growth, spore formation and germination of Bipolaris maydis could be inhibited by the metabolites. The mycelia affected by the metabolites also showed surface nodulation and protoplasmic condensation. It is speculated that the metabolites may target at the fungal cell walls.