猪繁殖与呼吸综合征(PRRS)抗体水平检测与分析

Estimation of Antibody in Vaccinated and Non-vaccinated Pig Farms with PRRS Vaccine

为探讨PRRS免疫猪场和非免疫猪场抗体水平差异,采集福建各地区64个不同规模猪场血清样本1 742份,其中免疫猪场40个共1 059份血清,非免疫猪场24个共683份血清;采用美国IDEXX公司PRRS病毒抗体ELISA试剂盒进行检测,分析其抗体阳性率、CV值及S/P区间分布值。结果显示:免疫猪场抗体阳性883份,阳性率算术平均值为83.4%,CV值为67.1,S/P值大于2.5的样本44份,占4.2%;非免疫猪场抗体阳性290份,阳性率算术平均值为42.5%,CV值为87.4,S/P值大于2.5的样本74份,占10.1%。表明免疫猪场抗体阳性率高,离散度小;非免疫猪场均可检测到PRRS阳性抗体,离散度大。揭示应加强PRRS的免疫,对疫情防控具有积极作用。

1059 serum samples from 40 swine farms of vaccination with PRRSV vaccine and 683 serum samples from 24 swine farms of non-vaccination with PRRSV vaccine were collected to determine antibody level of PRRSV with IDEXX PRRSV antibody ELISA Kit.Positive rate,coefficient variation（CV） and Sample/Positive（S/P） were analyzed.The result showed that in vaccinated pig farms,883 of 1059 serum samples were positive of PRRSV antibody,average positive rate and CV were 83.4% and 67.1,respectively;S/P of 44 serum samples was higher than 2.5,accounting for 4.2%.In non-vaccinated pig farms,290 of 683 serum samples were positive of antibody of PRRSV,average positive rate and CV were 42.5% and 87.4,respectively;S/P of 74 serum samples was higher than 2.5,accounting for 10.1%.The results indicated that non-vaccinated pig farms have been infected by PRRSV.Average positive rate of PRRSV antibody is higher in vaccinated pig farms than in non-vaccinated pig farms.Simultaneously,CV and S/P in vaccinated pig farms decreased obviously compared to non-vaccinated pig farms.It is concluded that vaccination with PRRSV vaccine is one of better ways to control PRRS.