脂肪酸在饲料原料油脂掺假辨析中的应用

Applications of fatty acids in the analysis of adulterated feedstuffs-oil

样品选用氢氧化钾-甲醇快速酯化法进行前处理,采用毛细管气相色谱法测定深海鱼油、大豆油、菜籽油、鸡油、猪油中特征脂肪酸,并对深海鱼油进行部分掺假试验,探讨了掺假鱼油中脂肪酸的变化趋势。掺假实验表明,掺假后鱼油脂肪酸含量随掺入油脂特征不同有不同的变化。当掺假量在10%以上时,EPA+DHA等特征指标变化趋势较明显,可作为油脂掺假鉴别的依据。

We pretreated the materials by potassium hydroxide-methanol rapid esterification method, and determined the characteristic patterns of fatty acid in deep-sea fish oil, soybean oil, rapeseed oil, chicken fat and lard oil using capillary gas chromatography, and discussed the variation trend of fatty acids in adulterated fish oil through part of deep-sea fish oil for the adulteration test. The adulteration experiments showed that fatty acids in fish oil after adulteration changed with different characteristics of mixing oil. When the amount of adulteration was more than 10%, the variation trends of EPA/DHA and other indicators were more obvious, which could be identified as the basis for oil adulteration.