摘要
目的分析INK4/ARF基因座甲基化状态与非小细胞肺癌临床病理特征的相关性及探讨基因座内各编码基因甲基化协同调控的可能性。方法临床收集81例非小细胞肺癌患者手术切除的配对癌灶及癌旁石蜡组织标本,采用甲基化特异性PCR对石蜡组织标本DNA进行p16INK4a、p15INK4b及p14ARF基因甲基化检测,并分析其与临床病理特征的关系。结果癌灶组织中的p16INK4a基因甲基化在鳞状细胞癌中的分布明显高于腺癌(P<0.01),并与肿瘤大小密切相关(P<0.05)。癌灶组织中p16INK4a与p15INK4b基因甲基化状态密切相关(P<0.05)。结论 INK4/ARF基因座启动子区甲基化与非小细胞肺癌发生、发展相关,p16INK4a与p15INK4b基因甲基化状态的关联提示INK4/ARF基因座可能存在表观协同调控方式。
Objective To investigate the possibility of coordinated methylation regulation of INK4/ARF locus and its correlation with clinical characteristics of non-small cell lung cancer.Methods Paraffin-embedded tumor and paracancerous non-tumor tissue samples,which were surgically resected from 81 non-small cell lung cancer patients,were collected.Methylation status of promoter of p16INK4a,p15INK4b and p14ARF were quantified with methylation-specific PCR（MSP）.Correlation between INK4/ARF locus methylation and clinicopathological features were statistically accessed.Results Significantly higher methylation of p16INK4a promoter was revealed in squamous cell carcinoma than that in adenocarcinoma（P0.01）,and was significantly correlated with tumor size（P0.05）.Meanwhile,significant correlation between p16INK4a and p15INK4b methylation in tumor tissue was observed（P0.05）.Conclusion Methylation of INK4/ARF locus is correlated with oncogenesis of non-small cell lung cancer.Correlation between p16INK4a and p15INK4b methylation implies that INK4/ARF gene locus may be coordinately and epigenetically regulated during development and progression of lung cancer.
出处
《广东医学》
CAS
CSCD
北大核心
2011年第19期2527-2530,共4页
Guangdong Medical Journal
基金
国家自然科学基金资助项目(编号:30860319)
国家大学生创新性实验计划项目(编号:081040314)
江西省卫生厅科技计划项目(编号:20072030)
