BDNF基因修饰真皮多能干细胞移植对脊髓损伤的修复作用

Role of transplantation of BDNF gene-modified dermal multipotent stem cells in repairment of spinal cord injury

目的观察脑源性神经营养因子(BDNF)基因修饰的真皮多能干细胞(dMSCs)移植后对损伤脊髓的修复作用。方法 将72只大鼠采用改良Allen重物打击法制备脊髓损伤模型,将其随机均分为A组[接受BDNF腺病毒表达载体(Adv-BD-NF)感染的dMSCs移植]、B组(接受未感染Adv-BDNF的dMSCs移植)、C组(单纯Adv-BDNF注射)和D组(单纯脊髓损伤)。免疫荧光组织化学检测脊髓损伤局部BDNF的表达,流式细胞仪检测损伤脊髓局部细胞凋亡情况,BBB(Basso,Beattie and Bresnahan locomotor rating scale)神经行为学评分法评价受伤大鼠神经功能恢复情况。结果 A组和C组大鼠损伤脊髓局部BDNF表达高于B组和D组;与B、C、D组比较,A组脊髓损伤部位的细胞凋亡率最低,神经功能恢复最好。结论 BDNF与dM-SCs移植可协同促进损伤脊髓功能的恢复。

Objective To observe the repair effects of transplantation of dermal multipotent stem cells（dMSCs）modified by brain-derived neurotrophic factor（BDNF）gene on spinal cord injury.Methods 72 rats was used to establish spinal cord injury models with improved Allen′s falling strike method,and then they were randomly divided into 4 groups：group A[accepted transplantation of dMSCs infected by adenovirus vector of BDNF（Adv-BDNF）],group B（accepted transplantation of dMSCs not infected by Adv-BDNF）,group C（injection with only Adv-BDNF）and group D（only spinal cord injury）.Immunofluorescence histochemistry and flow cytometry were employed to detect the expression of BDNF and apoptosis of cells in the site of spinal cord injury,respectively.BBB neurological behavior score method was used to assess neurofunctional recovery of injuried rats.Results Expression of BDNF in the site of spinal cord injury in group A and C were higher than those in group B and D;Compared with group B,C,D group,group A showed the lowest percentage of apoptotic cells in the site of spinal cord injury and the best neurofunctional recovery.Conclusion BDNF and dMSCs transplantation can synergistically promote the functional recovery of injuried spinal cord.