银杏叶提取物对大鼠重症急性胰腺炎肾损伤的保护作用及机制研究

The study on the protective effect and mechanism of ginkgo extract to the kidney injury of rats with SAP

目的探讨银杏叶提取物对大鼠重症急性胰腺炎(severe acute pancreatitis,SAP)肾损伤的保护作用及相关机制。方法 雌性SD大鼠90只,随机分为假手术组(N组),重症急性胰腺炎模型组(P组),银杏叶提取物治疗组(S组)。P组和S组采用5%牛磺胆酸钠逆行胰胆管注射方法复制大鼠重症急性胰腺炎模型,于12、244、8 h时间点对各组大鼠肾脏损伤进行大体形态和光镜下观察,测定大鼠血清尿素氮(blood urea nitrogen,BUN)、肌酐(crea,Cr)、肿瘤坏死因子-α(tumor necrosis factorα,TNF-α)和白细胞介素-6(interleukin-1,IL-6)的含量,并检测肾脏组织中的Toll样受体4(TLR4)和核因子κB(NF-κB)蛋白表达。结果 各时间点大鼠肾脏组织病理学评分、血清中BUN、Cr、TNF-α和IL-6的含量以及肾脏组织中TLR4和NF-κB的表达P组高于N组,S组低于P组。结论 银杏叶提取物对大鼠SAP肾损伤有保护作用,可能抑制TLR4/NF-κB信号通路中TLR4和NF-κB的活化,从而抑制下游炎症介质的释放来发挥保护作用。

Objective To study the protective effect and the possible mechanism of ginkgo extract on the kidney injury of rats with SAP.Methods 90 female Sprague-Dawley rats were randomly divided into sham operation group（N group）,severe acute pancreatitis group（P group）,extractive of ginkgo treatment group（S group）,respectively.5% sodium taurocholate was retrogradely injected into the pancreatic duct of rats in P and S groups to induce SAP.Rats of every group were respectively and humanely killed at 12 h,24 h,48 h to compare the kidney injury in every group for general morphology and light microscopy.Blood was obtained for BUN,Cr.TNF-αand IL-6 level detecting by ELISA,TLR4 and NF-κB in the kidney tissues were semi-quantified detected by immunohistochemical method.All the results were analysed by Anova with SPSS16.0.Results The injury of kidney tissues in P group was obvious,pathological scores were higher than those in N group.So were the concentrations of BUN、Cr、TNF-αand IL-6 and the expressions of TLR4 and NF-κB in the kidney tissues.All the results in S group were lower than those in P group.Conclusion The extraction of ginkgo has satisfactory therapeutic effect on kidney injury of rats with SAP depending on the activation inhibition of TLR4 and NF-κB.