北五味子有效成分的薄层光密度法测定

DETERMINATION OF THE ACTIVE INGREDIENTS IN CHINESE DRUG WUWEIZI (SCHISANDRA CHINENSIS) BY TLC-DENSITOMETRY

本文报道了用薄层光密度法测定北五味子种子中七个有效成分:五味子甲素、乙素、丙素、醇甲、醇乙、酯甲(乙)、五味子酚的含量。生药用己烷冷浸提取后进行薄层层析(硅胶GF_(254)薄层板)。展开剂:Ⅰ.甲苯-乙酸乙酯(9:1);Ⅱ.甲苯—乙酸乙酯(4:6),波长λ_S=260 nm,λ_R=350nm,反射法直线扫描。本法灵敏度高、重现性好。

A method of TLC densitometry was developed to determine the active ingredients (Wuweizisu A, B, C; Wuweizichun A, B; Wuweizi ester and schisanhenol) in Schisandra kernels. The samples were extracted with hexane(3×5ml) for 8, 4 and 4 h respectively. The hexane extract was evaporated to dryness and then dissolved in 1 ml of methanol. Standard solutions and sample solutions were spotted on a silica gel GF_(254) plate, and developed with toluene-ethyl acetate(9:1) for 18 cm (for Wuweizisu A, B, C) and. with toluene-ethyl acetate (4:6) for 18 cm (for Wuweizichun A, B, Wuweizi ester and schisanhenol). On examining the chromatogram under UV light the ingredients appeared as dark spots. A Shimadzu TLC model 910 was Used for scanning at λs 260 nm and λ_R 350 nm by reflection mode. Linear calibration curves Were obtained for the 7 constituents in the range of 1～5 μg. The average recoveries of the 7 constituents were 92.4～101.60%; the variation coefficients were 1.62～5.28%. The spots were stable for more than 24 h. This method is sufficiently simple, rapid and accurate for routine analysis.