小鼠尾动脉条应用于α肾上腺素受体激动剂、拮抗剂和钙拮抗剂的研究

APPLICATION OF MOUSE TAIL ARTERY FOR THE STUDY OF ALPHA ADRENOCEPTOR AGONISTS AND ANTAGONISTS AND CALCIUM ANTAGONISTS

近年内,国外一些学者已证明大鼠尾动脉存在突触后α_1和α_2肾上腺素受体并对此两种亚型的生理性质进行了研究、比较。目前,大鼠尾动脉条已广泛用于α_1及α_2受体激动剂和拮抗剂以及钙拮抗剂的生理和药理学研究。该方法需将动脉剪成螺旋条,制做比较复杂。为易于掌握,我们在制备方法上进行了改进。随后,设计和建立了小鼠尾动脉条实验模型。本文重点介绍小鼠尾动脉实验方法的优点及其应用范围,翼能在国内得到推广应用。

Isolated mouse tail artery strip was used for the study of α_1-,α_2-adrenoceptor agonists and antagonists. NA (α_1 and α_2 agonist) was shown to have greater activty in contracting tail artery. Phenylephrine (α_1 agonist) and clonidine (α_2 agonist) exhibited the same contractile action but much weaker than NA. Prazosin(α_1 antagonist) and yohimbine (α_2 antagonist) greatly diminished the contraction induced by phenylephrine and clonidine. These results indicate that mouse tail artery is rich in postsynaptic α_1-and α_2-adrenoceptor. In addition, mouse tail artery preparation was shown to be a useful tool for screening calcium agonists and antagonists. This model has advantages of being simple and easy to prepare, short equilibrium time and more economic in comparison with the helical strips of isolated rat tail artery.