乙型肝炎患者血清中抗原与病毒DNA关系探讨

The relationship between serum HBeAg and HBV-DNA in the patients infected HBV

目的探讨乙型肝炎(下称乙肝)患者血清中乙肝病毒e抗原(HBeAg)、乙肝病毒表面抗原(HBsAg)与乙肝病毒(HBV)DNA之间的关系。方法选取723例HBsAg阳性的乙肝患者血清标本,分别用微粒子酶免疫分析法(MEIA)检测HBeAg、HBsAg,用核酸扩增荧光定量聚合酶链反应法检测HBV-DNA。结果 HBeAg阳性标本中,HBV-DNA阳性率为60.9%;HBeAg阴性标本中,HBV-DNA阳性率为37.19%,二者差异有统计学意义(χ2=19.11,P<0.01)。不同浓度组HBeAg和HBV-DNA检测结果比较,差异有统计学意义(2χ=36.67,P<0.01)。不同载量HBV-DNA组HBeAg含量相差显著,HBV-DNA载量高,HBeAg含量也高,但HBsAg量差异无统计学意义。结论 HBsAg的浓度对乙肝的诊断治疗价值,HBeAg仍是反映乙肝复制活跃的一个可靠的指标,但是HBeAg阴性组HBV-DNA的阳性率为37.19%,说明仅检测HBeAg还是不够的,同时检测HBeAg、HBV-DNA对乙肝患者的判断、治疗方案的选择和疗效判断有一定的指导意义。

Objective To explore the relationship between serum HBeAg and HBV-DNA contents.Methods The HBeAg,HBsAg levels of 723 serum samples with positive HBsAg were tested by microparticle enzyme immunoassays（MEIA）and HBV-DNA was tested by fluorescence quantitative polymerase chain reaction（FQ-PCR）.Results In the HBeAg positive samples,the positive percentage of HBV-DNA was 60.9%,and in the HBeAg negative samples,the positive percentage of HBV-DNA was 37.19%,which showed significant difference（χ^2=19.11,P〈0.01） between HBeAg and HBV-DNA.The differences between HBeAg in a series of concentration and the test results of HBV-DNA were also significant（χ^2=36.67,P 0.01）.Conclusion Compared with HBeAg,HBV-DNA is a better marker for detecting the replication of HBV,which would be helpful for the HBV clinical therapy.