3种常用溶液配制ABO反定型红细胞的探讨

Discussion of the three common solution on preparation of red blood cell suspension for reverse ABO typing

目的对3种常用溶液配制ABO反定型红细胞悬液进行比较。方法利用生理盐水、低离子溶液、Sysmex血液分析仪用稀释液3种溶液制备2%的红细胞悬液,(4±2)℃冰箱保存,每天对红细胞的凝集强度、红细胞数量和质量、上清液游离血红蛋白进行观察,每3天监测一次细菌生长情况等。观察用3种溶液制备的反定型红细胞的有效期限。结果用Sysmex血液分析仪专用稀释液配制的反定型红细胞有效期仅6 d,用生理盐水配制的反定型红细胞有效期不到24 h,低离子溶液配制的反定型红细胞当天溶血。结论用Sysmex血液分析仪专用稀释液可制备简便的ABO反定型红细胞,有效期6 d;低离子溶液不适用于配制的反定型红细胞;生理盐水配备的反定型红细胞应每天配制。

Objective To compare the three common solution on preparation of red blood cell suspension for reverse ABO typing.Methods We used three suspension including physiological saline,low ionic solutions,dilution of Sysmex hematology analyzers to prepare 2% liquid of red blood cell suspension,（4±2）℃ refrigerators preserved every day,and observed on the strength of agglutination of red blood cells,red blood cell volume and quality,the supernatant of free hemoglobin,then monitored the growth of bacteria one time every three days.We observed validity period of the three red blood cell suspensions for reverse typing.Results The validity periods of red blood cell suspension for reverse typing prepared by the dilution of Sysmex hematology analyzers and physiological saline and were 6 days and less than 24 hours,and the suspension prepared by low ionic solutions could only be used in that day.Conclusion The dilution of Sysmex hematology analyzers may prepare simple reverse ABO typing of red blood cells,low ionic solution does not suitable for the preparation,and the suspension prepared by saline should be prepared every day.