免疫组化对形态学上难以分型的急性白血病的诊断价值

Clinical study of immunohistochemistry in acute leukemia that hard to be morphologically classified

目的探讨骨髓片免疫组化检查对急性白血病的诊断价值。方法对48例形态学难以分型的急性白血病进行免疫表型分析,与形态学诊断结果进行比较,并和流式细胞仪分型进行对照。结果 M0的形态学分型与免疫组化分型存在差异,经2χ检验,差异有统计学意义(P<0.05)。免疫组化分型与流式细胞仪分型有很好的一致性,较形态学诊断的正确率更高。结论骨髓片免疫组化对形态学难以分型的急性白血病分型中有较好的临床价值,能为临床提供更可靠的治疗依据。

Objective To discuss the value of bone marrow smear immunohistochemistry examination in the diagnosis of acute leukemia.Methods We did the immunophenotype analysis on 48 patients with acute leukemia that hard to be diagnosed morphologically,and compared it to the results of morphology diagnosis,with the flow cytometry（FCM） morphology as the control.Results There was statistically significant difference between the diagnosis morphology and immunohistochemistry morphology of M0 by χ^2 test（P〈0.05）.There was good consistency between the immunohistochemistry morphology and FCM morphology,with higher correctness rate than the morphology diagnosis.Conclusion The bone marrow smear immunohistochemistry examination has a high clinical value in the diagnosis of acute leukemia,which could provide more reliable treatment references.