摘要
本文用GC-ECD法测定血中美托洛尔浓度。美托洛尔及内标烯丙洛尔在四氢呋呋喃化下,能迅速、定量地转化为双-七氟丁酰化衍生物,经3% SE-30玻璃填充柱分离及ECD检测。本法线性范围10～1500 ng/ml(r=0.9993),美托洛尔的平均回收率97.30%,日内及日间差的变异系数分别小于7%租8%。本法已用于美托洛尔控释剂型的生物利用度及药代动力学研究,以及临床血药浓度监测。
A method is described to determine metoprolol in plasma by GC-ECD. Metoprolol and alprenolol (internal standard) reacted rapidly with heptafluorobutyric anhydride under the catalysis of tetrahydrofuran (THF)after being extracted from plasma with cyclohexane. The derivatives produced quantitatively were identified by GC-MSD to be diheptafluorobutyl metoprolol and diheptafluorobutyl alpredolol which were then separated and detected by 3% SE-30 packed glass column (2m×3mm)equipped with ECD. The detection limit was 1 ng/ml and the linearrangewas 10～1500 ng/ml (r=0.9993).Average recovery from spiked plasma was 97.30%. The coefficient of variation of day-to-day and within-day were less then 8% and 7%, respectively. The method has been applied to investigate the Pharmacokinetics and bioavailability of metoprolol and also applied to clinical monitoting of metoprolol levels in patients.
出处
《药学学报》
CAS
CSCD
北大核心
1990年第6期457-461,共5页
Acta Pharmaceutica Sinica
关键词
美托洛尔
GC-ECD法
Metoprolol
GC- ECD
Diheptafluorobutyric derivatives
