不同土壤pH对多叶羽扇豆花色影响研究

Study on the Effect of Flower Color of Lupinus polyphyllus in Different Soil pH

以5种不同土壤pH的蓝色多叶羽扇豆(Lupinus polyphyllus ‘Nanus Russell Gallery Blue’)为材料,对其花色、花色素组成及含量的变化进行了研究。花色测定采用英国皇家园艺学会比色卡(RHSCC)和分光色差计,色素定性及定量分析采用高效液相色谱-二极管阵列检测技术(HPLC-PAD)和分光光度计进行测定。结果发现:随着土壤pH的降低,花朵翼瓣亮度L*值、色相a*值、彩度C*值逐渐降低,色相b*值逐渐升高,总花青苷、叶绿素含量升高;旗瓣中总花青苷也逐渐升高,其他各检测结果无明显相关性。推断土壤酸碱性影响蓝色多叶羽扇豆花瓣中花青苷的含量,从而影响花色的深浅。

The changes of flower color,pigments composition of Lupinus polyphyllus ＇ Nanus Russell Gallery Blue ＇ in five soil pH were studied.The flower color was measured according to the Royal Horticultural Society Colour Chart（RHSCC） and by a NF333 spectrophotometer,respectively.A high performance liquid chromatography（HPLC） with a photodiode array detector（HPLC-PAD） and spectrophotometer were employed for qualitative and quantitative analysis of anthocyanins,flavonols,chlorophyll and carotenoids.The results indicated that the wing L ＊ 、 a ＊ 、 C ＊ reduce and b ＊ rise with soil pH reducing,total anthocyanins（TA） and Chlorophyll content increased.TA also increased in the banner,but others content no obvious relativity from different soil pH.That tentatively identified soil pH did effect accumulation of anthocyanins in flower,and effect flower color of L.polyphyllus ＇ Nanus Russell Gallery Blue ＇.