山野菜荚果蕨颈卵器发育的研究

Study on Archegonium Development of Matteuccia struthiopteris

对荚果蕨有性生殖方式的研究,有利于保持和培育该野生物种资源。主要利用光学显微镜和石蜡切片技术详细观察和记录了荚果蕨颈卵器的发育过程,结果表明:颈卵器是由原叶体表面细胞中颈卵器原始细胞发生,颈部由4～6层细胞组成;卵细胞的形成经历2次不均等分裂,腹沟细胞解体标志着卵细胞的成熟;顶端细胞打开,成熟的卵细胞进行受精;当颈卵器的腹沟细胞浓缩成为略扁的半球形结构而不解体,促使颈卵器败育。本研究首次阐明荚果蕨颈卵器内细胞的发育顺序和特征,为开发应用山野菜荚果蕨提供必要的参考资料。

Experiments based on sexual reproduction Matteuccia struthiopteris way to do further research,and helps keep and breeding wild species resources.Mainly by means of optical microscope and paraffin technology detailed observe and record the archegonium of Matteuccia struthiopteris development process,and the results showed that the archegonium formed in the under surface cell of the prothallium consists of 4-6 cells in the neck;Egg formation through two not equal division,the ventral canal cell disintegrated marks mature egg;Apical cell open,mature oocytes were fertilized;When the ventral canal cell concentrate became slightly flat hemispherical structure and non-dismatiement,prompting archegonium abortion.This study was the first time to specify the sequences and characteristics of the archegonium interior cell in Matteuccia struthiopteris,provided necessary reference material for the development and application of wild Matteuccia struthiopteris.