用ROC曲线比较TRFIA和CLIA检测AFP在PHC诊断中的价值

目的:分别用时间分辨荧光免疫分析(TRFIA)和CLIA法检测血清AFP浓度,比较两种检测方法对原发性肝癌(PHC)诊断的临床应用价值。方法:选择门诊和住院期间得到确诊的原发性肝癌患者114例和肝良性病变患者126分别用TRFIA和CLIA法检测受试者血清AFP浓度,并进行工作特征(ROC)曲线分析。结果:以正常对照组95%区间为医学正常值范围,TRFIA法和CLIA法诊断肝癌的临床界限值分别为7.31ng/mL和24.17ng/mL。TRFIA诊断肝癌的灵敏度为46.9%,特异性为93.4%,诊断准确率为79.2%。CLIA法的灵敏度为41.2%,特异性为91.6%,诊断准确率为65.8%。两种检测方法ROC曲线下面积分别为0.761(TRFIA)和0.682(CLIA)。结论:TRFIA和CLIA法都可以满足临床AFP检测需要,但TRFIA法优于CLIA法。

Objective： To determine the levels of AFP in serum by Time - resolved Abstract：To evaluate the clinical value of the Time - resolved immunofluo massay（TRFIA） and Chemiluminescent immunoassay（CLIA） methods in the determination of serum AFF levels for the diagnosis of primara hepatic ca rcinoma（PHC）. Methods： one hundred and fourteen patients with PHC and 126 patients with liver benign disease （LBD）were included in this study. The serum levels of AFP were determined bv RFI and CLIA,respeetively.The results from serum samples from the patients with PHC and LBD groups were used to ealeulate ROC.Results： The eut off values of serum AFP levels for TRFIA and CLIA were set at 7.31ng/ml and 24.17ng/ml, respeetivelv.The diagnostic specificity and sensitivity were 46.9% and 93.4% for TRFIA,and 41.2% and 91.6% for CLIA respectively.The area under the ROC curve for TRFIA and CLIA was 79.2% and 65.8%, respectively. Conclusions： The results indicate that both TRFIA and CLIA could meet the clinical diagnostic needs and TRFIA is superior to CLIA for identifying patients with PHC.