胎儿真皮间充质干细胞中的全能性相关转录因子的表达

Detection of pluripotent transcription factors in fetal dermis-derived mesenchymal stem cells

背景:人真皮纤维母细胞或间充质干细胞可形成诱导性多能干细胞,但不同研究者所用的转录因子组合却并不相同。目的:分离人胎儿真皮间充质干细胞,检测其全能性相关转录因子的表达。方法:水囊引产5月龄胎儿,按照既往分离培养间充质干细胞的方法,得到胎儿真皮间充质干细胞。结果与结论:胎儿真皮间充质干细胞高表达Oct4和C-myc、中度表达Sox2,是形成诱导性多能干细胞较好的体细胞。

BACKGROUND： The induced pluripotent stem （iPS） cells have been reprogrammed from human dermal fibroblasts and mesenchymal stem cells （MSCs）. Various combinations of transcription factors are used by different investigators. OBJECTIVE： To detect the pluripotent transcription factors expressed in fetal dermis-derived MSCs. METHODS： Using previous methodology in isolation and cultivation of MSCs, fetal dermis-derived MSCs were obtained from 5-month-old fetus, inducing abortion by water bag. RESULTS AND CONCLUSION： Transcription factors of Oct4 and C-myc were expressed highly and Sox2 was expressed moderately. These results suggest that fetal dermis-derived MSCs are better somatic cells for iPS cells.