脐血间充质干细胞静脉移植治疗新生鼠脑血肿继发脑水肿

Effects of intravenous transplantation of umbilical blood mesenchymal stem cells on cerebral edema secondary to hematoma in neonatal rats

背景:脐血间充质干细胞移植治疗新生动物脑血肿继发脑水肿的研究少见报道。目的:探讨脐血间充质干细胞静脉移植治疗新生鼠实验性脑血肿继发脑水肿的可行性。方法:健康新生7dSD大鼠分为未进行干预的空白对照组、仅制作脑出血模型的脑血肿对照组和制作脑出血模型后给予脐血间充质干细胞治疗的移植组。3组大鼠均于移植后1周处死,提取脑组织进行指标检测。结果与结论:脑血肿形成后,脑系数和脑组织含水量均明显增加,脐血间充质干细胞移植组明显小于脑血肿对照组(P<0.01),与空白对照组比较差异无显著性意义(P>0.05)。移植1周后,各组鼠死亡率无显著差异,均未见植入反应和其他任何负作用。提示,脐血间充质干细胞静脉移植治疗能有效减轻实验性脑血肿继发的脑水肿,对其具有明显的保护作用。其机制可能与脐血间充质干细胞能有效地透过血脑屏障,在病灶脑组织周围迁移、扩散、整合,从而在病灶脑组织存在并分泌大量保护性细胞因子有关。

BACKGROUND： There are few reports about human umbilical cord blood mesenchymal stem cells （HUCBMSCs） for cerebral edema secondary to hematoma in neonatal animals. OBJECTIVE： To study the possibility of intravenous transplantation of HUCBMSCs in the treatment of cerebral edema secondary to hematoma in neonatal rats. METHODS： Neonatal rats aged 7 days were randomly assigned into three groups： normal control, model group and MSCs transplantation group. One week after transplantation, the rats in each group were randomly killed to extract brain tissues which were used pathomorphology observation and index measurement. RESULTS AND CONCLUSION： Both brain coefficient and water content of brain tissue were significantly lower in the MSCs group than the model group （P 0.01）. However, there was no difference between MSCs group and control group （P 0.05）. There was no difference in the death rate of rats in each group 1 week after the implantation. The brain damage to most of the rats in this group was not serious but only slight by chance, close to the normal at the one week after implantation. HUCBMSCs transplantation could effectively decrease the brain edema and ameliorate the damaged brain tissue. One week after transplantation, the MSCs appeared in the ischemic damaged brain tissue as a large number of MSCs could cross the blood brain barrier to distribute and scatter around the disease focus integrated with host brain tissue without apparent threshold.