体外高浓度葡萄糖培养及黄芪干预人外周血内皮祖细胞的数量和增殖与分化

Effects of high glucose and Astragalus Membranaceus on the number, proliferation, and differentiation of endothelial progenitor cells from human peripheral blood in vitro

背景:内皮祖细胞数量及功能受损是糖尿病血管并发症发生发展的重要环节,黄芪对多种原因引起内皮功能障碍具有保护作用,可以有效保护血管内皮功能。目的:观察高浓度葡萄糖及黄芪干预对人外周血内皮祖细胞数量、增殖及分化影响。方法:不同浓度葡萄糖孵育内皮祖细胞24h以及30mmol/L葡萄糖孵育内皮祖细胞不同时间;内皮祖细胞经20g/L黄芪预处理24h后,再加入30mmol/L葡萄糖培养24h。结果与结论:高葡萄糖以浓度及时间依赖方式减少内皮祖细胞的数量,降低内皮祖细胞的增殖及向内皮细胞系分化能力(P0.05或0.01),给予黄芪预处理后,内皮祖细胞数量明显增加,增殖及向内皮细胞系分化能力明显增强(P<0.05或0.01)。提示高葡萄糖以浓度及时间依赖方式减少外周血内皮祖细胞数量,降低内皮祖细胞的增殖及向内皮细胞系分化能力,黄芪可以改善高葡萄糖对内皮祖细胞的损伤作用。

BACKGROUND： The quantity and impaired function of endothelial progenitor cells （EPCs） is the important link of diabetic vascular complications. Astragalus Membranaceus （AMI） can protect endothelium of dysfunction which caused by various reasons and can effectively protect the function of vascular endothelium. OBJECTIVE： To investigate the effects of high glucose and AMI on the number, proliferation, and differentiation of EPCs from human peripheral blood in vitro. METHODS： EPCs were incubated with various concentrations of glucose for 24 hours and with 30 mmol/L glucose for different periods. Simultaneously, EPCs were pre-incubated with 20 g/L AMI for 24 hours and then all the cells were incubated with 30 mmol/L glucose for 24 hours. RESULTS AND CONCLUSION： High glucose decreased the number of EPCs and abilities of EPCs proliferation and differentiation in a dose- and time-dependent manner （P 0.05 or 0.01）. Pre-incubated with AMI could increase EPCs quantity and improve EPCs proliferative and differentiative capacity （P 0.05 or 0.01）. AMI can increase the number and improve the capacity of proliferation and differentiation of EPCs cultured with high glucose.