人参皂苷Rg1干预骨髓间充质干细胞转化为多潜能干细胞关键基因mRNA的表达

Effects of Ginsenoside Rg1 on the expressions of Nanog, c-Myc, Oct, Klf4, Sox2 mRNA during the differentiation of bone marrow mesenchymal stem cells into pluripotent stem cells

背景:将成体细胞重编程为诱导多潜能干细胞方案主要通过反转录病毒将Oct-4,Sox2,c-Myc,Klf4等基因转入成体细胞而实现。目的:观察人参皂苷Rg1作用于骨髓间充质干细胞后,对成体细胞向诱导多潜能干细胞转化的关键性基因Oct4、Sox2、c-Myc、Klf4、NanogmRNA表达的影响。方法:培养骨髓间充质干细胞,对照组培养基为α-MEM,体积分数5%FBS,1%双抗;用药组培养基为α-MEM,体积分数15%FBS,1000U/mLRatESGRO,1%双抗,并加入6.25μmol/L和12.5μmol/L人参皂苷Rg1。检测骨髓间充质干细胞Oct4,Sox2,c-Myc,Klf4,Nanog等mRNA的表达。结果与结论:人参皂苷Rg16.25μmol/L培养30d,Nanog、c-Myc、Oct、Klf4、Sox2mRNA表达均有升高,且Nanog、c-Myc与对照组差异有显著性意义。人参皂苷Rg1能促进骨髓间充质干细胞表达c-Myc,Nanog,但Nanog阳性的诱导多潜能干细胞在基因表达谱上很难与胚胎干细胞区分出来,提示人参皂苷Rg1对骨髓间充质干细胞向诱导多潜能干细胞转化可能具有促进作用。

BACKGROUND： Somatic cells are reprogrammed to induced pluripotent stem cells （iPSCs）, mainly through anti-retroviral program to transfer Oct-4, Sox2, c-Myc, Klf4 and other genes into somatic cells. OBJECTIVE： To observe the effects of Ginsenoside Rg1 on Oct4, Sox2, c-Myc, Klf4, Nanog mRNA expression during differentiation of bone marrow mesenchymal stem cells （BMSCs）. METHODS： The BMSCs were cultured; 6.25 μmol/L and 12.5μmol/L Ginsenoside Rg1 of Chinese medicine extraction were added into culture medium, respectively. Oct4, Sox2, c-Myc, Klf4, Nanog mRNA expressions were detected in BMSCs. RESULTS AND CONCLUSION： In the BMSCs cultured by 6.25 μmol/L Ginsenoside Rg1 for 30 days, the expressions of Nanog, c-Myc, Oct, Klf4, Sox2 mRNA of BMSCs were increased, and the expression of the gene Nanog and c-Myc had significantly difference compared with the control group. It demonstrated that Ginsenoside Rg1 could enhance the expressions of Nanog, c-Myc, which are crucial genes on converting the BMSCs to iPS. But Nanog positive iPS cells are very difficult to be discriminated from the embryonic stem cells in gene expression profile; therefore, we consider that Ginsenoside Rg1 might facilitate the differentiation from BMSCs to iPS.