脂肪干细胞的体外诱导与分化

In vitro induction and differentiation of adipose-derived stem cells

背景:应用脂肪干细胞来作为组织工程的种子细胞是近年来组织工程研究中的一个活跃领域,大量的研究表明在不同的条件下脂肪干细胞可以向机体的不同组织分化。目的:对国内外脂肪干细胞体外诱导分化的现状及新进展作一综述。方法:应用计算机检索CNKI和PubMed数据库中2000-01/2010-10关于脂肪干细胞的文章,在标题和摘要中以"脂肪干细胞,体外分化潜能,种子细胞,转基因"或"Adipose-derived stem cells,celldifferentiation in vitro,seeded cell,genetrans faction"为检索词进行检索。选择文章内容与脂肪干细胞体外分化有关者,同一领域文献则选择近期发表或发表在权威杂志文章。初检得到112篇文献,最终入选28篇文献进行综述。结果与结论:脂肪干细胞是存在于脂肪组织中的具有高度自我更新能力和多向分化潜能的干细胞群体。它具有多向系分化潜能,除可以分化为间充质来源的脂肪、骨、软骨以及骨骼肌、心肌等细胞,也可诱导分化为来源于外胚层的神经细胞以及具有功能性的血管内皮细胞。此外脂肪干细胞还具有造血支持作用以及可被腺病毒等高度转染等优点,可以作为基因转移良好的靶细胞。

BACKGROUND： For the past few years, the application of adipose derived stem cells （ADSCs） as seeded cells of tissue engineering is an active field in medications and ADSCs could differentiate into many tissues in vitro. OBJECTIVE： To summarize the situation and progression about ADSCs induced in vitro at home and abroad. METHODS： Databases of CNKI and PubMed （2000-01/2010-10） were selected to search the related articles about ADSCs using theof ＂adipose-derived stem cells, cell differentiation in vitro, seeded cell, gene transfaction＂ in Chinese and English. There were 112 articles after the initial survey. Then 28 articles related to sustained-release antimicrobials were involved. RESULTS AND CONCLUSION： ADSCs have extensive self-renewal capacity and the ability to differentiate along multiple lineages. With the multilineage differentiation potential, ADSCs can differentiate not only into such mesenchymal lineages as adipogenesis, chondrogenesis, osteogenesis, myogenesis, but also into neurogenesis of origin ectoderm. In addition, ADSCs have high infected efficiency by oncoretroviral vectors and have the ability to support hematopoiesis.