丹黄通络胶囊对老年痴呆大鼠学习记忆能力及脑内胆碱能系统的影响

Effect of Danhuangtongluo capsule on learning and memory ability and cholinergic system of Alzheimer′s disease rats

目的观察丹黄通络胶囊对阿尔茨海默病(AD)模型大鼠学习记忆能力及脑内胆碱能系统的影响。方法采用β淀粉样蛋白(Aβ25-35)侧脑室注射制备AD大鼠模型,用Morris水迷宫实验检测大鼠空间学习记忆能力,采用比色法测定大鼠脑组织中胆碱乙酰转移酶(ChAT)和乙酰胆碱酯酶(AchE)的活性,并应用免疫组化法检测海马神经元M1受体的表达。结果丹黄通络胶囊组大鼠经治疗后,Morris水迷宫实验成绩明显优于模型组(P<0.05);且脑组织中AchE活力明显降低,ChAT活力明显增强,海马神经元细胞膜上M1受体的平均灰度也明显降低,与模型组相比差异均具有统计学意义(P<0.01),且呈现一定剂量依赖性。结论丹黄通络胶囊对AD模型大鼠的学习记忆能力减退具有一定改善作用,其作用机制可能与保护中枢胆碱能系统有关。

Objective To observe the effect of Danhuangtongluo capsule on learning and memory ability and cholinergic system in the brain of Alzheimer′s disease（AD） model rats.Methods AD model rats were established by injected Aβ25~35 to lateral ventricle.Morris water maze test was used to measure the learning and memory function.The activities of choline acetyltransferase（ChAT） and acetylcholinesterase（AchE） in the brain were examined by colorimetry.The expression of M1 receptor in hippocampus was measured with immunohistochemistry.Results Danhuangtongluo capsule improved learning and memory ability of AD model rats,and not only obviously decreased AchE activity and increased ChAT activity,but also the expression of M1 receptor in hippocampus was improved.Conclusions Danhuangtongluo capsule can effectively improve cognitive performance in AD model rats,which maybe related to protect the central cholinergic system.