摘要
目的:建立HPLC法测定桃红前列康丸中丹参酮ⅡA含量的方法。方法:采用Eclipse XDB-C18色谱柱,甲醇—水(75:25)为流动相;流速0.6ml•min-1;检测波长270nm。结果:丹参酮ⅡA在17.2~154.8μg范围内线性关系良好(r=0.9998),平均回收率为98.48%,RSD=1.02%(n=6)。结论:本方法操作简便,专属性强,结果准确,可用于桃红前列康丸的质量控制。
Objective: To establish a HPLC method to determination of Tanshinone Ⅱ A in taohongqianliekang pill. Method: The chromatographic system consisted of C18column,using methanol -waters(75 : 25) as mobile phase.The content was detected at a waveleng of 270 nm,the flow rate was 0.6ml·min^-1 .Results: The Tanshinone Ⅱ A have a good tincar rolatim in the range of 17.2-154.8 μ g ( r=-0.9998 ) ,the average recovery was 98.48% and RSD=1.02% ( n=6 ) . Conolusion; The established method is accuracy ,sensitive and simple.It can be used for quality control of taohongqianliekang pill.