一个新的水稻花器官突变体的鉴定和基因定位

Characterization and Gene Mapping of a Novel Mutant in Rice Floral Organs

在水稻(Oryza sativa)品种台中65的组培后代中发现一个花器官发育异常突变体flower organ number6(fon6),其主要表型为:双子房,多柱头,7-8枚雄蕊。遗传分析表明,该突变表型由一对隐性基因控制。以该突变体与籼稻3037杂交的F2代分离群体作为定位群体,利用STS标记将与突变性状相关的基因定位于第6染色体短臂上STS标记PL4和PL5之间约480kb的范围内。该研究结果为进一步的基因克隆及功能研究奠定了基础。

We obtained a rice floral mutant,inner flower organ number 6（fon6）,from the progenies of tissue culture of a japonica variety Taichung65.The mutant has double ovaries,multi-stigmas,and 7 to 8 stamens.The mutant trait is controlled by a single recessive gene.The F2 population derived from the cross between Zhongxian 3037 and fon6 was used for gene mapping.FON6 was mapped to the ~480-kb region between the sequence-tagged site markers PL4 and PL5 on chromosome 6.These results will be useful for cloning and functional analysis of the gene.