月季愈伤组织的诱导及植株再生

Callus Induction and Plant Regeneration of Rose

以可在黑龙江地区露地越冬的5个现代月季(Rosa chinensis)品种为实验材料,分别以其无菌苗的叶片和茎段为外植体,研究了愈伤组织诱导及植株再生方法。实验结果表明:5个寒地月季品种的叶片和茎段均可诱导出愈伤组织,2,4-D诱导愈伤组织的效果较好,高浓度的细胞分裂素不适合用于月季叶片和茎段愈伤组织的诱导;TDZ在月季愈伤组织分化培养过程中具有重要作用,光照培养可促进月季愈伤组织的分化,愈伤组织的分化能力随着继代次数的增加呈下降趋势。该实验成功地从2004-8和2004-9(2个月季品种)愈伤组织中诱导出再生植株,其愈伤组织的分化率分别为45%和38%。

We used the leaves and stems of aseptic seedlings of 5 rose cultivars that can successfully live outside through the winter in Heilongjiang Province as explants to investigate callus induction and plant regeneration.The leaves and stems of the 5 rose cultivars could induce callus;media containing 2,4-D could induce callus better;and a high concentration of cytokinin was not suitable for callus induction.TDZ played an important role in callus differentiation;culture under light conditions was effective for callus differentiation;and callus differentiation decreased with increasing number of subcultures.Plant regeneration could be successfully induced from the callus of 2004-8 and 2004-9 in experimentation,and the callus differentiation rate of the 2 varieties was 45% and 38%,respectively.