外周血Lunx mRNA、Muc1 mRNA、CEA联合检测对非小细胞肺癌微转移的诊断价值

Diagnosis of micrometastases by detection of Lunx mRNA,Muc1 mRNA and CEA in peripheral blood of patients with non-small cell lung cancer

目的检测非小细胞肺癌(non-small cell lung cancer,NSCLC)患者外周血中肺特异性X蛋白(LunxmRNA)、黏蛋白基因1(Muc1 mRNA)及CEA,探讨其对NSCLC微转移的诊断价值及临床意义。方法 72例NSCLC癌患者、10例肺良性疾病患者及12例健康志愿者,以逆转录-聚合酶链反应(reserve transcriptase-polymerasechain reaction,RT-PCR)方法,对其外周血进行Lunx mRNA和Muc1 mRNA的检测;以免疫化学发光测定法,检测其血清的CEA水平。结果 Lunx mRNA和Muc1 mRNA在NSCLC患者外周血中的表达率与肺良性病变组和正常对照组比较差异有统计学意义(P<0.01);其表达与NSCLC组织分化程度和临床分期相关(P<0.05),Muc1 mRNA的表达还与组织学类型有关(P<0.05)。以多种指标多种方法联合检测阳性率为最高。结论 Lunx mRNA和Muc1 mRNA是检测NSCLC微转移的良好指标。两者联合CEA检测可以提高肺癌检出率。

Objective To detect the expression of Lunx mRNA,Muc1 mRNA and CEA in peripheral blood of patients with non-small cell lung cancer,and to explore the clinical and diagnostic value to the micro-metastasis of NSCLC. Methods RT-PCR was used to detect the Lunx mRNA and Muc1 mRNA in peripheral blood from 72 patients with NSCLC,10 patients with benign lung diseases and 12 healthy volunteers were served as controls.Serum of CEA was measured by electrochemiluminescence. Results Positive rate of Lunx mRNA and Muc1 mRNA in peripheral blood of patients with NSCLC was significant different compared with the other two groups（P0.01）,the expression of Lunx mRNA was related with histological grade and clinical stage of NSCLC（P0.05）,while Muc1 mRNA was not only related with these,but also with the histological type（P0.05）.Combined with a variety of methods,the rate of positive detection can be highest. Conclusion Lunx mRNA and Muc1 mRNA are smart detections for micrometastasis of NSCLC,and combined with CEA could improve the positive rate of NSCLC.