B2 SINE RNA和HEXIM1蛋白相互作用的研究

Study of the interaction between B2 SINE RNA and HEXIM1 protein

构建了带FLAG多肽标签的hHEXIM1(HMBA-inducible protein 1)蛋白真核表达载体,在人胚胎肾细胞(human embryonic kidney 293 cells,HEK293 cells)中检测蛋白表达正确后,将该质粒转入小鼠的成纤维细胞(NIH3T3细胞)中,进行anti-FLAG的免疫沉淀(im-munoprecipitation,IP)实验.之后用半定量逆转录PCR(reverse transcriptase PCR,RT-PCR)的方法证明了hHEXIM1蛋白是可以和B2 SINE RNA相互作用的.并由此提出了关于B2SINE RNA和HEXIM1蛋白共同参与调控基因表达调控的新假设.

A FLAG peptide tagged hHEXIM1 protein expression plasmid was constructed. After expression ensured in HEK293 cells, the plasmid was tranfected into the NIH3T3 cells, then an anti-FLAG immunoprecipitation was carried out. The interaction between B2 SINE RNA and hHEXIM1 protein was figured out by semi-quantitative reverse transcriptase PCR （RT-PCR） followed. Given that, a hypothesis that B2 SINE RNA and HEXIM1 protein regulate the gene expression in the same pathway was proposed.