响应面法优化叶酸高产菌发酵培养基及发酵条件

Optimization of Culture Medium and Fermentation Conditions for Highly Folic Acid Production by Response Surface Methodology

采用响应面法对叶酸产生菌产朊假丝酵母Y2.12的发酵发酵培养基及发酵条件进行优化.首先,采用Plackett-Burman方法对影响发酵各因素的效应进行评价,筛选出有显著效应的3个因素:碳源乳糖、pH值和摇床转速;并通过Box-Benhnken设计和响应面分析法对这3个主要影响因素进行分析.结果表明,优化后这3个因素的最佳值为摇床转速196r/min、pH7和碳源乳糖含量为6.25%.采用优化后的发酵培养基及发酵条件进行摇瓶发酵,叶酸的含量可达23.14±0.13mg/L,取得了很好的优化效果.

The response surface methodology was used to optimize the culture medium and fermentation conditions of Candida utili Y2.12.In the first step of optimization,carbon source-corn flour,pH and the speed of shaker and were found to be the important factors by Plackett-Burman design.Then,the optimal value of three important factors were determined by Box-Benhnken design and response surface methodology.The result was the speed of shaker 196 r/min,pH 7 and 6.25%corn flour.After the optimization,the yield of folic acid increased markedly compared with the initial and reached 23.14±0.13 mg/L.