apoB基因EcoRI、XbaI位点和apoAI基因-75bp、＋83bp位点多态性的联合作用与哈萨克族人群血脂异常的关系

Association of apolipoprotein B gene EcoRI and XbaI and apolipoprotein AI gene - 75 bp and ＋ 83 bp polymorphisms with dyslipidemia in Kazaks

目的分析载脂蛋白B（apoB）基因EcoRI、XbaI位点和载脂蛋白AI（apoAI）基因一75bp、＋83bp位点多态性与哈萨克族人群血脂异常的关系。方法采用聚合酶链式反应-限制性片段长度多态性分析法检测225例哈萨克族血脂异常患者和187例哈萨克族正常对照者各个位点多态性，并分析其多种联合与血脂异常之间的关系。结果（1）两组各基因型联合的总体分布不同（P〈0．05），基因型联合7频率在两组之间比较（19．1％vs11．2％）差异有统计学意义（P〈0．05）。（2）血脂异常组中基因型联合7的患者apoB、甘油三酯、总胆固醇水平显著高于其余基因型联合（联合1～6）的平均水平，apoAI、apoAI／apoB水平显著低于其余基因型联合的平均水平；同时此类患者的LDL—C显著低于基因型联合4，apoAI／apoB显著高于基因型联合4；而且其apoAI／apoB、HDL-C显著低于基因型联合1。（3）对照组基因型联合7的HDL-C、apoAI／apoB显著低于基因型联合1—6的平均水平，LDL—C显著高于基因型联合1～6平均水平；其HDL．C、apoAI／apoB显著高于基因型联合4；apoAI／apoB显著低于基因型联合1。结论基因型联合7与新疆石河子地区哈萨克族人群血脂异常相关，其机制可能与基因变异引起apoB、apoAI／apoB的变化等有关，且血脂紊乱可能始于基因变异引起的apoAI／apoB的变化。基因型联合4可能与哈萨克族人群血脂异常相关，基因型联合1可能预防血脂异常的发生。

Objective To explore the relationship between polymorphisms of EcoRI,XbaI of apolipoprotein B （apoB）gene and -75 bp, ＋ 83 bp of apolipoprotein AI （apoAI） gene and dyslipidemia in Ka-zaks of Shihezi district, Xinjiang, China. Methods These polymorphisms were analyzed by PCR-PFLP. Results （1） The ratio of Coalition 7 is higher in dyslipidemia group compared with control group （ 19.1% vs 11.2%, P 〈 0.05 ）. （ 2 ） Coalition 7 in dyslipidemia group is associated with increased apoB, TC,TG and decreased apoAI, apoAI/apoB compared with the others as an unit （ Coalition 1 - 6 ） of the same group significantly;its LDL-C is lower and apoAI/apoB is higher than Coalition 4 significantly;and both of its apoAI/apoB and HDL-C are lower than Coalition 1 of the same group significantly. （ 3 ） Coalition 7 in control group is associated with decreased apoAI/apoB,HDL-C and increased LDL-C compared with Coalition 1-6 significantly;its apoAI/apoB, HDL-C are higher compared with Coalition 4; and its apoAI/apoB is lower than Coalition 1 of the same group significantly. Conclusion It is thought that Coalition 7 is related with dyslipidemia in Kazaks of Shihezi through increased apoB and decreased apoAI/apoB, and that dyslipidemia is initiated from altered apoAI/apoB. Perhaps Coalition 4 is also related with dyslipidemia and Coalition 1 is a protective style for dyslipidemia.