应用SRAP标记分析福瑞鲤及其原始亲本的遗传结构

Analysis of Genetic Structure of FFRC Strain Common Carp (Cyprinus carpio) and Its Original Parents by SRAP Markers

应用SRAP分子标记对建鲤(Cyprinus carpio var.jian)、黄河鲤(C.c.haematopterus)和福瑞鲤(FFRC Strain Common Carp,C.carpio)进行遗传结构分析。结果显示,筛选出的10个多态性较好的引物组合共扩增出110个位点,其中多态性位点92个,平均多态性比率83.6%,平均多态性信息含量为0.256。对3个鲤鱼群体的Nei's基因多样指数、Shannon信息指数和群体多态位点比例等遗传参数进行了分析,3个鲤鱼群体的Nei's基因多样指数分别为0.221、0.205和0.233;Shannon信息指数分别为0.332、0.298和0.352;群体多态位点比例分别为68.1%、65.2%和71.2%。结果表明,福瑞鲤群体遗传多样性水平较其原始亲本高,具有较丰富的遗传多样性,因而该群体仍具有一定的选育潜力。遗传相似度和遗传距离结果表明,福瑞鲤继承了更多建鲤的遗传物质。

SRAP markers were used to amplify and analyze the genomic DNA of three Common Carp populations including Jian Carp（Cyprinus carpio var.jian）,Huanghe Carp（C.c.haematopterus）,and the FFRC Strain Common Carp（C.carpio）.Of the 110 bands amplified by 10 primer combinations,92（83.6%） were polymorphic.The average of polymorphism information content（PIC） was 0.256.The highest and lowest Nei＇s diversity indexes were found in FFRC Strain Common Carp population（0.233） and Jian Carp population（0.205）,respectively.The average Shannon＇s indexes were 0.332,0.298 and 0.352,respectively in three populations.As to the proportion of polymorphic loci,the most diverse group examined in our work was the FFRC Strain Common Carp population（71.2%）,and the least was Huanghe Carp population（65.2%）.The results indicate rich polymorphism information content and large genetic diversity in the FFRC Strain Common Carp population.The genetic distance and genetic identity results also suggest that the FFRC Strain Common Carp inherits more genetic material from Jian Carp than from Huanghe Carp.