异丙酚对油酸型急性肺损伤兔血清TNFα-、IL-1β水平的影响

Effect of propofol on serum TNF-α and IL-1β in rabbit with acute lung injury induced by oleic acid

目的:观察异丙酚对油酸性急性肺损伤(ALI)兔血清TNFα-、IL-1β水平的影响,探讨异丙酚治疗油酸型ALI的可能机制。方法:健康日本大耳白兔24只,随机分成对照组(Ⅰ组)、急性肺损伤组(Ⅱ组)和异丙酚治疗组(Ⅲ组)。Ⅱ、Ⅲ组兔静脉注射油酸0.08 mL/kg,复制日本大耳白兔ALI模型。建模成功后Ⅲ组静脉注射异丙酚8 mg.kg-1.h-1,Ⅰ组和Ⅱ组静脉注射生理盐水0.8 mL.kg-1.h-1至实验结束。各组大耳白兔分别在输入异丙酚或生理盐水前(T0)和给药后1 h(T1)、2 h(T2)、3 h(T3)、4 h(T4)采集动脉血用ELISA法检测血清TNF-α、IL-1β,同时进行血气分析,计算PaO2/FiO2结果:Ⅰ组血清中TNF-α、IL-1β浓度各时点无明显变化(P>0.05)。Ⅱ组TNFα-浓度在T1开始升高,T2达高峰,T3、T4有下降但仍高于T0(P<0.05),与Ⅰ组相应时点比较升高明显(P<0.05)。Ⅲ组各时点TNF-α浓度升高明显低于Ⅱ组(P<0.05)。与T0比较,Ⅱ组血清IL-1β浓度在T1开始升高,T4达高峰(P<0.05),各时点浓度均明显高于Ⅰ组(P<0.05),Ⅲ组IL-1β浓度升高程度低于Ⅱ组(P<0.05)。Ⅰ组各时点氧合指数无明显变化。Ⅱ、Ⅲ组随着时间延长氧合指数逐渐下降,明显低于Ⅰ组(P<0.05),Ⅲ组氧合指数下降幅度较Ⅱ组低(P<0.05)。结论:异丙酚能通过降低油酸性ALI兔血清TNF-α、IL-1β的释放,提高油酸性ALI后氧合指数,对兔油酸性ALI起到一定的保护效果。

AIM： To observe the effects of propofol on serum TNF-α,IL-1β in rabbit with acute lung injury（ALI） induced by oleic acid（OA） and explore its possible protective mechanism and provide evidence for the choice of anesthesia drug in clinical anesthesia.METHODS： Twenty four health Japanese white rabbits were randomly divided into three groups（n=8）： control group（group Ⅰ）,model group（groupⅡ） and propofol treatment group（group Ⅲ）.The rabbits in group Ⅱ and Ⅲ were injected oleic acid（OA）（0.08 mL/kg） by marginal veins of ear to establish ALI models.The rabbits of group Ⅲ were injected propofol（8 mg·kg-1·h-1） with pump,while group Ⅰ and group Ⅱ were injected normal saline（0.8 mL·kg-1·h-1） to end of the experiment after establishing the ALI models successfully.The concentration of TNF-α and interleukin-1β in serum were detected by ELISA at the points of propofol or normal saline infusion（T0）,1 h after administration（T1）,2 h after administration（T2）,3 h after administration（T3）,4 h after administration（T4）,and blood gas analysis of them had been performed to calculate the PaO2/FiO2 Ratio.RESULTS：The concentrations of TNF-α and interleukin-1β in serum in group Ⅰ had no difference at different points（P0.05）.The concentration of TNF-α began to increase at T1,reached to peak at T2 and decreased at T3,T4,but still higher than those of T0（P0.05）.The concentrations of TNF-α in group Ⅱ increased more obviously than those of group Ⅰ（P0.05） and were lower in group Ⅲ than those of group Ⅱ（P0.05） at the corresponding time point.Compared with T0 the concentrations of interleukin-1β in groupⅠbegan to increase at T1,reached to the peak at T4（P0.05） and were higher than those of group Ⅰ at the corresponding time points（P0.05）,increased lowerly in group Ⅲ than those of group Ⅱ（P0.05）.The oxygenation index at different time points had no significant change in group Ⅰ,decreased gradually after establishing the ALI models successfully in group Ⅱ and Ⅲ,were lower in group Ⅱ and Ⅲ than those of group Ⅰ（P0.05）,while decrease less in group Ⅱ than group Ⅲ.CONCLUSION： Propofol can significantly decrease the concentrations of serum TNF-α and IL-1β and obviously improve the oxygenation index of rabbits with ALI,has a possible protective effect of ALI induced by oleic acid.