3种不同种植体表面特征及骨结合力的比较分析

Comparison of Surface Morphologies and Implant-bone Integrate Strength

目的:通过喷砂酸蚀(SLA)、微弧氧化(MAO)、微弧氧化-浸提续断粗提取液(MAO-Radix Dipsac)在钛合金TLM表面制备3种涂层,测定骨结合力值比较3种涂层的效果。方法:使用扫描电子显微镜、能谱分析仪(EDS)比较涂层的形貌;选取6只杂种犬,每侧股骨植入3种试件(直径3.3mm,长度11mm)各1颗。术后4、8、12周分别处死2只犬取股骨,充分显露种植体两端。使用WDT-10拉伸试验机推出种植体,记录最大值。SEM观察被推出种植体的表面形貌。结果:不同时间和浓度续断浸提钛合金MAO表面,发现孔内出现不同程度的沉积,沉积物质成分主要为C、O、Mg、S元素;ANOVA分析种植体-骨推出结果显示,3组每个月的实验力值间分别存在统计学差异。MAO组、MAO-Radix Dipsac组在4、8、12周与SLA组的实验力值有显著差别(P<0.05);MAO组与MAO-Radix Dipsac组在4、8周实验力值有显著差别(P<0.05),而12周时两者无显著差异。结论:3种方法处理的种植体均能达到骨结合,MAO-Radix Dipsac组、MAO组比SLA组达到更快而强的骨结合力值;MAO-Radix Dipsac组比MAO组达到更快的骨结合。

Objective： To observe three different surface coatings on TLM alloy formed by SLA,MAO,MAO-dipping into extract of radix dipsac,respectively,and to evaluate the bone response effects of them.Methods： Surface properties of these implants were observed with SEM and EDS.Each kind of implants formed by SLA,MAO,MAO-Radix Dipsac were implanted into each femur of 6 dogs.Two animals were sacrificed in 4,8 and 12 weeks respectively.The implant-femur resin blocks were harvested immediately and were incised to expose both ends of the implants and got a flat-bottomed surface that was parallel to the implant platform.Biomechanical stability and interface character were assessed by methods of push-out testing to obtain the push-out values and SEM scanning to observe surface properties after push-out testing.Results： Dipped into extract of Radix dipsac（1.25g/mL and 2.5g/mL of concentration） for different time after MAO,some substance whose mainly elements were C,O,Mg and S,were deposited on the surface.To lengthen in duration,surface holes were filled in various degrees.ANOVA analysis revealed push-out values were statistical different in every dealing time of each group.Besides,Dunnett-t analysis revealed there was statistical difference between SLA group and MAO group,MAO-Radix Dipsac Radix group in 4,8 or 12 weeks of healing time（P0.05）.However,there was no statistical difference between MAO group and MAO-Radix Dipsac Radix group in 12 weeks of healing time.Conclusion： All implants got osteointegration.MAO-Radix Dipsac group and MAO group got more rapidly and strongly integration with bone than SLA group.MAO-Radix Dipsac group got the fastest osteointegration.