胃癌外周血淋巴细胞亚群表达水平对患者生存率的影响

Impact of lymphocytes subgroups in peripheral blood on survival rate of patients with gastric cancer

目的探讨胃癌患者外周血淋巴细胞亚群表达与生存率的关系。方法用流式细胞仪检测833例胃癌首诊患者外周血的淋巴细胞亚群CD3^＋、CD4^＋、CD8^＋、CD4^＋／CD8^＋、CD19^＋、CD25^＋、CD44^＋及NK^＋细胞．并根据96名健康对照者的平均检测值分为高表达组和低表达组．比较各亚群高表达组与低表达组患者的生存率。结果与健康对照者相比．胃癌患者CD3^＋、CD8^＋低表达。而CD4^＋,CDl9^＋,CD25^＋、CD4^＋／CD8^＋、CD44^＋,NK^＋高表达（P〈0．05）。CD19^＋高表达与低表达者分别为444例和389例，3年生存率为36．4％和18．5％，差异有统计学意义（P〈0．05）：而其他7种淋巴细胞亚群表达水平则与患者生存率无关（均P〉0．05）。结论与健康人群相比，胃癌患者外周血淋巴细胞亚群发生显著变化．其中CDl9^＋高表达患者具有明显的生存优势。

Objective To study the change of lymphocyte subgroups in the peripheral blood of patients with gastric carcinoma and its association with survival. Methods Flow cytometry was used to examine the subgroups of lymphocytes （CD3^＋, CD4^＋, CD8^＋, CD4^＋/CD8^＋, CD19^＋, CD25^＋, CD44^＋ and NK cells） in the peripheral blood of 833 gastric carcinoma patients prior to any therapy. Patients were divided into the high expression group and lower expression group according to the average test values of 96 healthy control subjects. Survival rate was compared between the two groups. Results Compared with control group, the levels of CD3^＋ and CD8^＋ T cell in patients were significantly lower, while the levels of CD4^＋, CD19^＋, CD25^＋, CD4^＋/CD8^＋, CD44^＋, and NK^＋ cells were significantly. The differences were statistically significant （P〈0.05）. Three-year survival rates of gastric cancer patients with high CD19^＋ expression （n=444） and cases with low CD19^＋ expression （n=389） were 36.4% and 18.5%, respectively （P〈0.05）. The expressions of other seven types of lymphocytes were not associated with survival rates （all P〉0.05）. Conclusions Significant changes in lymphocyte subgroups exist in the peripheral blood of patients with gastric carcinoma. Patients with high CD19^＋ expression have better survival.