染色法鉴别旋毛形线虫成虫死活的实验观察

Experimental study on determination of viability of the adult of Trichinella spiralis in vitro

目的:探讨快速鉴别旋毛形线虫成虫死活的染色方法。方法:分别以0.5%番红、1.0%中性红和0.5%甲基蓝3种染液,对死或活旋毛形线虫成虫进行染色。结果:0.5%番红、1.0%中性红染液能使死亡旋毛形线虫成虫着色;旋毛形线虫活成虫对3种染液均不着色。结论:0.5%番红、1.0%中性红染液染色均可快速鉴别旋毛形线虫成虫死活。

Objective：To explore the prompt methods in determination of viability of the adult of Trichinella spiralis.Methods：The adult worms of Trichinella spiralis were stained with 0.5% safranine,1.0% neutral red or 0.5% methylene blue.Results：The dead adults were observed when stained with 0.5% safranine or 1.0% neutral red in the study.The vital adults were unstained with all three stains.Conclusions：Both 0.5% safranine staining and 1.0% neutral red staining are prompt method in determination of viability of the adult of Trichinella spiralis.