α-半乳糖苷酶法合成α-半乳糖基-β-环糊精的研究

Enzymatic Synthesis of α-Galactosyl-β-cyclodextrin by α-galactosidase

应用发芽咖啡豆α-半乳糖苷酶法合成α-半乳糖基-β-环糊精,研究其合成工艺。通过Box-Behnken设计等方法,得出工艺条件为α-半乳糖苷酶用量73 nkat,时间28 h,pH 6.3,温度40℃,振荡速度75 r/min,反应体系包括2 mL醋酸缓冲液(50 mmol/L),β-环糊精0.4 mol/L,蜜二糖0.8 mol/L。在此条件下,α-半乳糖基-β-环糊精绝对产率为28.4%,比优化前提高了41.3%。

Parameters affecting the synthesis of α-galactosyl-β-cyclodextrin by α-galactosidase of germinating coffee beans were systematically studied by Box-Behnken design. The optimized conditions were as follows, enzyme concentration, 73 nkat; Reaction time, 28h; pH, 6.3; Reaction temperature, 40℃; Oscillatory speed, 75 r/min; β-cyclodextrin, 0.4mol/L; melibiose, 0.8mol/L; and acetate buffer （50 mmol/L）, 2 mL, The yield of α-galactosyl-β-cyclodextrin was 28.4% under these conditions, which was 41.3% higher than conditions before optimization.