水稻基因OsASIE1抗逆功能分析

Function Analysis of Gene OsASIE1 Responding to Abiotic Stresses in Rice

作为重要的植物转录因子家族,AP2/EREBP转录因子在植物发育、激素、病原反应及非生物胁迫如干旱、高盐、低温应答方面起重要作用。本研究发现水稻AP2/EREBP转录因子家族EREBP亚家族成员OsASIE1(abiotic stress induced EREBPgene)在水稻受到高盐、干旱胁迫时表达量迅速提高,并且在水稻中超表达OsASIE1能够改善水稻抵抗盐胁迫的能力。凝胶迁移率检测(electrophoresis mobility shift assay,EMSA)表明,该转录因子的AP2结构域能够结合干旱应答顺式作用元件DRE(dehydration-responsive element)和乙烯应答元件GCCbox(ethylene response element),推测OsASIE1可能通过结合DRE和GCCbox作用元件调控下游相关基因的表达,进而调控相关抗逆反应。

AP2/EREBP transcription factors play an important role in plant development, hormone response, biotic and abiotic stress responses. We identified that OsASIE1, a member of EREBP subfamily of AP2/EREBP transcription factor family in rice, was involved in abiotic stress response. The expression of OsASIE1 was induced by drought and salt stresses, and over-expression of OsASIE1 in the transgenic rice plant could improve its tolerance to salt stress. Further electrophoresis mobility shift assay （EMSA） revealed that the AP2 domain of OsASIE1 protein could bind both DRE （dehydration-responsive element） and GCC box （ethylene response element, ERE） in vitro. All these results implicated that OsASIE1 might be involved in abiotic stress response by regulating the expression of downstream genes with DRE and GCC box binding.