星点设计—效应面法优选两面针活性成分的提取工艺

SELECTION ON THE OPTIMAL EXTRACTION OF ZANTHOXYLUM NITIDUM(ROXB.)DC BY CENTRAL COMPOSITE DESIGN-RESPONSE SURFACE METHODOLOGY

目的:优选两面针活性成分的最佳提取工艺。方法:以加氯仿倍量、提取时间为主要影响因素,以氯化两面针碱、乙氧基白屈菜红碱和L-芝麻素含量为评价指标,用星点设计—效应面法优选两面针活性成分的最佳提取工艺。结果:最佳提取工艺为加入60倍量氯仿,提取3次,每次提取120min。结论:用星点设计—效应面法优选两面针活性成分的提取工艺简便易行,预测性好。

Objective： To optimize the extracting technology of active ingredients. Methods： The main influencing factors of extraction effect included the quantity of chloroform and extraction time and the extraction conditions were evaluated by the contents of niticline chloride, ethoxychelerythrine and L-sesamin. The optimal extraction of active ingredients was selected by central composite design-response surface methodology. Results：The optimal conditions consisted of extraction three times, 120 rain each time with sixty fold of chloroform. Conclusion：It is simple, convenient and high predictive to optimize the extraction craft of active ingredients by central composite design-response surface methodology.