白癜风进展期患者皮肤疱液中NPY、sICAM-1、IL-12和GM-CSF水平变化的实验研究

Experimental Study of Changes of Skin Blister Fluid NPY,IL-12,sICAM-1 and GM-CSF Levels in Patients with Vitiligo in Progressive Stage

目的:探讨白癜风病患者的发病、进展与免疫学机制的关系。方法:将确诊为进展期白癜风的80例患者分为寻常型(54例),节段型(26例)组。各以白斑处和非白斑处2组的疱液中的研究指标水平进行比较。疱液NPY和GM-CSF采用RIA;IL-12s、ICAM-1水平均采用ELISA。结果:本文测定的患者疱液NPY水平显示,寻常型白癜风患者白斑处与非白斑处无显著差异(P>0.05),节段型白癜风患者白斑处与非白斑处比较则呈显著性差异(P<0.05)。IL-12s、ICAM-1和GM-CSF三项指标寻常型白癜风患者白斑处与非白斑处比较则均呈显著性差异(P均<0.05)。节段型患者则显示白斑处与非白斑处比较无显著差异(P>0.05)。结论:白癜风病患者的发病及病情进展与疱液NPY、IL-12s、ICAM-1和GM-CSF四项指标的变化关系密切,其测定有助于了解其病因及病理学机制。

Objective To explore the significance of changes of skin blister fluid NPY,IL-12,sICAM-1 and GM-CSF levels in patients with vitiligo in progressive stage.Methods 80 patients with vitiligo in progressive stage were divided into two groups（vulgaris vitiligo groups： n=54,segmental vitiligo groups： n=26）Their blister fluid levels of NPY and GM-CSF were determined by radioimmunoassay（RIA）,and IL-12 and sICAM-1 were determined by enzyme immunoassay.Results The levels of skin blister fluid NPY were definitely higher in vitilignous skin than those in non-vitilignous patches in segmental vitiligo groups（P0.05）,while in vulgaris vitiligo groups were mutually not significant difference（P0.05）.The levels of skin blister fluid IL-12,sICAM-1 and GM-CSF were all obviously higher in vitilignous skin than that in non-vitilignous patches in vulgaris vitiligo groups（P0.05）,while in segmental vitiligo groups were mutually all not significant difference（P0.05）.Conclusion The changes of skin blister fluid NPY,IL-12,sICAM-1 and GM-CSF levels in vitilignous skin may be closely related to development of difference type vitiligo patients with vitiligo,determination of 4 indexes might be helpful for studying the pathogenesis and clinical diagnosis of vitiligo.