降解毒死蜱多功能菌株的构建及其对土壤的修复作用

Construction of multifunctional bacterium strain of chlorpyrifos degradation and its bioremediation application

将实验室保存的有机磷降解酶基因mpd连接到自杀性质粒pUT-Km1上,构建重组质粒pUT-M,通过三亲杂交,将mpd整合到越南伯克氏菌B418的染色体上,获得多功能工程菌株。实验证明工程菌株的原有解磷、解钾、固氮活性没有改变;48 h后对溶液中毒死蜱降解率达到65%;对室内模拟污染土壤有一定的降解能力,20 d对土壤中的毒死蜱降解率达91%。

We constructed recombinant plasmid pUT-M by ligating mpd gene on a suicide plasmid pUT-Kml. We integrated mpd gene onto the chromosome of B. vietnamiensis B418 by triparental hybridization and obtained a multifunctional engineering stain. Experimental results show that the engineering stain retains its original activity of phosphorus or potassium decomposition and nitrogen fixation. The strain degrades chlorpyrifos in solution at a rate of 65% 48 hours later. Moreover, the strain can also degrade chlorpyrifos in simulated soil and the degradation rate can reach 9]% 20 days later.