福建地区汉族人群骨保护素基因rs2073617T/C多态性与急性冠脉综合征病变程度的关系

Correlation between osteoprotegerin gene rs2073617T/C polymorphism and severity of acute coronary syndromes in Fujian area

目的探讨福建地区骨保护素(osteoprotegerin,OPG)基因启动子rs2073617T/C位点基因多态性与急性冠脉综合征(acute coronary syndrome,ACS)病变程度的关系。方法采用多聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)技术检测360例ACS患者和120例正常对照者OPG基因rs2073617T/C多态性位点基因型,同时采用DNA测序对酶切产物进行鉴定,并比较其与ACS及其病变枝数和冠状动脉积分的关系。结果 OPG基因rs2073617T/C多态性TC、TT、CC三种基因型在ACS组和对照组中的频率分别为43.3%、33.3%和23.3%,以及47.7%、26.7%和25.6%;两组比较差异无统计学意义(P>0.05);ACS患者冠状动脉单枝、双枝、多枝病变组间OPG基因rs2073617T/C基因型频率差异无统计学意义(P均>0.05)。结论 OPG基因rs2073617T/C多态性与福建地区汉族人群中ACS的发生及冠状动脉病变程度无相关性。

Objective To detect the association of osteoprotegerin gene rs2073617T/C polymorphisms with severity of acute coronary syndrome（ACS） in Fujian area.Methods The polymerase chain reaction restriction fragment length polymorphism（PCR-RFLP） was used to detect OPG gene rs2073617T/C in three hundred and sixty patients with ACS（cases group） and 120 matched control group.DNA sequences of enzyme digested products were also analyzed.Results The frequencies of TC,TT and CC types of OPG rs2073617T/C polymorphism in ACS patients and controls were 43.3%,33.3% and 23.3% VS 47.7%,26.7% and 25.6%.There was no significant difference in the distribution frequency of three genotypes and two alleles of OPG rs2073617T/C between the ACS and control groups（P〉0.05）.OPG gene rs2073617T/C in all lesions was no difference among the groups（P〉0.05）.Conclusion OPG gene polymorphism was existing in Hans population of Fujian Han,and have no association with acute coronary syndrome and severity of ACS in Han nationality of Fujian.