复方奥硝唑-甲磺酸培氟沙星缓释制剂对人牙周膜细胞凋亡及超微结构的影响

Effect of Compound Ornidazole-pefloxacin Mesylate Sustained Release Preparation of Human Periodontal Ligament Cell Apoptosis and Ultrastructure Expression

目的:观察不同浓度复方奥硝唑-甲磺酸培氟沙星缓释制剂对人牙周膜细胞(HPDLC)凋亡与超微结构的影响。方法:用含有不同浓度复方奥硝唑-甲磺酸培氟沙星缓释制剂(0、1.25、2.5、5、10、20g/L)的培养液对人牙周膜细胞进行培养,流式细胞术检测细胞凋亡指数;透射电镜下观察HPDLC超微结构的改变。结果:在1.25、2.5 g/L的浓度下对HPDLC的凋亡率和超微结构均与对照组无显著性差异;而5、10 g/L的浓度组的细胞凋亡率较对照组小且超微结构显示细胞胞质内粗面内质网和线粒体增多,细胞突起增多;而在高浓度药物20 g/L的作用下,凋亡率有所增加,细胞发生退变,溶酶体增多。结论:5、10 g/L的复方奥硝唑甲磺酸培氟沙星缓释制剂对体外培养的HPDLC的生长有一定促进作用,高浓度药物20g/L则对HPDLC生长有一定的抑制作用。

Objective： To investigate the effect of different concentrations of compound ornidazole-pefloxacin mesylate sustained release preparation on human periodontal ligament cells （HPDLC） apoptosis and ultrastructure expression. Methods： Flow cytometry was used to detect the apoptosis rate of the HPDLC in different concentrations of compound ornidazole-pefloxacin mesylate sustained release preparation. The cell ultrastructure was detected by transmission electron microscope （TEM）. Results： In 1.25, 2.5g/L group, HPDLC apoptosis and ultrastructure had no significant difference with that in the control group; In 5,10g/L concentration of the apoptosis rate group was smaller than that in the control group and the ultrastructure showed rough endoplasmic reticulum and mitochondria increased significantly in cytoplasm; The action of HPDLC under the high concentrations of drug in the 20g/L, the apoptosis rate increased compared with that in the control group, and cell degeneration, lysosomes increased. Conclusion： 5,10g/L of compound ornidazole pefloxacin mesylate sustained release preparation can promote the in vitro growth of HPDLC, 20g/L drug concentration can inhibit the growth of HPDLC.